Dr Shinya Yamanaka of the Centre for iPS Cell Research and Application for Kyoto University received the Nobel Prize in which gas played an important role.
As reported in The Gas Review, iPS utilises the cells of patients or human cells. First of all these are frozen, preserved, and then stores in a cell bank. Liquid Nitrogen (LIN) is used for the freezing and preservation. When they are used for experiments, they are warmed in a water bath at a temperature of 37 oc, and thawed. These iPS cells are sowed in a flask containing a culture medium, and are then cultivated in a carbon dioxide (CO2) incubator.
A liquid nitrogen dewar is used as the cell bank. When the cells are frozen, they are frozen in stages in a program freezer. A small amount of liquid nitrogen is used here too to prevent cell destruction..
The carbon dioxide incubator is a required item for not only iPS cell research but also biotechnology research using human and animal cells. Around two to four cylinders of carbon dioxide are required per annum for one carbon dioxide incubator. This is just to add additional carbon dioxide to replace that which is emitted when the incubator is opened.